DNA detection made easy - GoFuckYourself.com

Juicy D. Links · 04-06-2004, 11:23 PM

Physicists in France have shown that it is possible to detect DNA with a purely electronic technique. Ulrich Bockelmann and colleagues at the Ecole Normale Supérieure in Paris measured the intrinsic charge of DNA molecules with an array of silicon transistors, which allowed them to avoid the markers and labels used in conventional detection techniques. The team has already used its device to detect and identify a common genetic mutation in human DNA, and hopes to exploit the approach in lab-on-a-chip applications (F Pouthas et al. 2004 Appl. Phys. Lett. 84 1594).

Bockelmann and colleagues exploited the fact that most biological molecules are charged in solution by building an array of 100 field-effect transistors (FETs) that were spaced tens of microns apart. Each transistor had an active surface area of tens of microns squared and was covered by a layer of silicon dioxide 10 nanometres thick.

The group placed the biomolecules on the surface of the array and measured the electronic properties of each transistor in the network. DNA molecules produce a negative shift in potential between the source and drain electrodes in the transistors because they are negatively charged in aqueous solution. By measuring the size of this potential shift, it is possible to identify the molecules in the solution.

Bockelmann and co-workers demonstrated the sensitivity of the technique by detecting and identifying a genetic mutation called 35delG that is responsible for hereditary deafness. The detection of specific mutations relies on the use of a polymerase chain reaction to increase the size of the sample.

jimboc · 04-06-2004, 11:26 PM

Inventions like this scare me

twistyneck · 04-06-2004, 11:27 PM

Maybe this will help them find the French "surrender" gene.

Repetitive Monkey · 04-06-2004, 11:53 PM

Quote:

Originally posted by jimboc
Inventions like this scare me

You shouldn't be scared of technology. Only intent.

$5 submissions · 04-07-2004, 12:11 AM

Great post, Juicy. Lab on a chip... interesting. Definitely more streamlined than the current polymerase (sic?) process pioneered by Kari Mullis.

pimplink · 04-07-2004, 01:37 AM

woah, juicy, what happen to you? you've been
posting some serious threads, lately.

Gheenz · 04-07-2004, 01:39 AM

Quote:

Originally posted by twistyneck
Maybe this will help them find the French "surrender" gene.

LMFAO!!!!

reynold · 04-07-2004, 02:56 AM

Cool breakthrough!

torrey · 04-07-2004, 03:00 AM

Quote:

Originally posted by twistyneck
Maybe this will help them find the French "surrender" gene.

04-06-2004, 11:23 PM	#1
Juicy D. Links So Fucking Banned Industry Role: Join Date: Apr 2001 Location: N.Y. -Long Island -- Posts: 122,992	DNA detection made easy Physicists in France have shown that it is possible to detect DNA with a purely electronic technique. Ulrich Bockelmann and colleagues at the Ecole Normale Supérieure in Paris measured the intrinsic charge of DNA molecules with an array of silicon transistors, which allowed them to avoid the markers and labels used in conventional detection techniques. The team has already used its device to detect and identify a common genetic mutation in human DNA, and hopes to exploit the approach in lab-on-a-chip applications (F Pouthas et al. 2004 Appl. Phys. Lett. 84 1594). Bockelmann and colleagues exploited the fact that most biological molecules are charged in solution by building an array of 100 field-effect transistors (FETs) that were spaced tens of microns apart. Each transistor had an active surface area of tens of microns squared and was covered by a layer of silicon dioxide 10 nanometres thick. The group placed the biomolecules on the surface of the array and measured the electronic properties of each transistor in the network. DNA molecules produce a negative shift in potential between the source and drain electrodes in the transistors because they are negatively charged in aqueous solution. By measuring the size of this potential shift, it is possible to identify the molecules in the solution. Bockelmann and co-workers demonstrated the sensitivity of the technique by detecting and identifying a genetic mutation called 35delG that is responsible for hereditary deafness. The detection of specific mutations relies on the use of a polymerase chain reaction to increase the size of the sample.

04-06-2004, 11:26 PM	#2
jimboc Confirmed User Join Date: Jun 2002 Location: Internet of course Posts: 1,614	Inventions like this scare me __________________

04-07-2004, 12:11 AM	#5
$5 submissions I help you SUCCEED Industry Role: Join Date: Nov 2003 Location: The Pearl of the Orient Seas Posts: 32,195	Great post, Juicy. Lab on a chip... interesting. Definitely more streamlined than the current polymerase (sic?) process pioneered by Kari Mullis. __________________ Need a NON-ADULT income stream? We build your YOUTUBE CHANNEL for only $20 per video! For Hire: $3/hour or $400 per month Marketing Specialist Virtual Assistants

04-07-2004, 01:37 AM	#6
pimplink Confirmed User Join Date: Jun 2001 Location: Closer than you think Posts: 9,535	woah, juicy, what happen to you? you've been posting some serious threads, lately. __________________ Need Mainstream Content and SEO? SEO * Website Copy * Blogs Blogging - PR Work - Forum Marketing - Social Marketing - Link building - Articles 100% Guaranteed Content!

04-06-2004, 11:27 PM	#3
twistyneck So Fucking Banned Join Date: Jan 2002 Location: Hanging by the neck until dead. Posts: 4,660	Maybe this will help them find the French "surrender" gene.

04-07-2004, 02:56 AM	#8
reynold Too lazy to set a custom title Join Date: Oct 2002 Location: Global Traveler Posts: 51,271	Cool breakthrough!