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IS and low light have a subtle relationship. First, I don't think you can use IS with a tripod.
Second, if you intend not to use a flash (or external light), you can always get a good exposure, but turning down the shutter means that you will risk a subject with motion blurring.
You may not need a tripod at 1/40, depending on the length of the lense. Try a monopod first, you may be surprised with the results.
I have a Canon 20D, you will notice noise in ISO's of 800+.
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