A few years back we had a hive the size of a bowling ball among the rafters of the dead space in the attic. Occasionally they'd come through the wall into the office and annoy the fuck outta me and the cats.
So I sleuthed around and found an outside hole they were using to get in and out of the attic. I bought some foaming Raid, emptied half the can into the hole (at night), and then sealed it with expanding foam insulation (the aerosol can stuff).
It did the trick.
edit - should mention ours were yellowjackets, not honeybees. The nasty little fuckers with bad tempers.