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Young, it depends what color your background is ... are you using a plain background or do you want this graphic to be able to go onto any background? If you've got a light image with a white background and you want to put it onto a black background its really hard to get it perfect ... you could try making the background of your image a totally opposite color to your graphic and page background (ie lime green or bright pink) so you make sure to get every pixel when selecting the colors to make transparent.
If its for a static site with a solid background, make the background of your gif as close to the background of your page as possible. From the color reduction drop down menu in the 'save for web' window select 'adaptive', then select 'diffusion' from the dither menu, and 'diffusion transparency dither' from the transparency menu. I find that gives the smoothest edges.
You might have to save it a few times to see what works best by selecting different colors to make transparent ... just make sure the background of your image isn't the same color as any of the colors you want to keep in the image.
Hope that made some sense ;/
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