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Old 05-09-2014, 02:09 AM  
wehateporn
Promoting Debate on GFY
 
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HPV Vaccine Contaminant Confirmed by Independent Lab

This gets worse, but with $1 Billion a year at stake don't expect it to be pulled from the shelves

http://sanevax.org/gardasil-contamin...dependent-lab/


"Recently Lee SH showed that Gardasil® contained fragments of HPV-11 or HPV-18 DNA, evidenced by nested PCR, of unknown significance [J Inorg Biochem. 2012 Dec;117:85-92]. We herein looked by optimized single PCR in different batches of Gardasil® from France for HPV L1 DNA using MY09/MY11 degenerate and nondegenerate primers, for HPV E2 and E6 DNA genes, and for contaminating Saccharomyces cerevisiae DNA. All amplified amplicons were sequenced and resulting FASTA sequences were analyzed by Genotyping software from NCBI. In-house quantitative single PCR using as external calibrator serial dilutions of HPV-16 DNA extracted from CaSki cell line allowed estimating the load of residual HPV DNA fragments in vaccine ampoules. Preliminary data showed the presence of contaminating HPV L1 DNA in all tested different batches of Gardasil® vaccine from France. Our observations confirm independently and extend the previous observations by Lee SH, without using conflicting nested PCR detection possibly subjected to contamination."
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